- Home
- ...
- Search Current Opportunities
- Job detail
Description & Requirements
Description & Requirements
The Huang Lab at UCSF (https://huanglabucsf.com/) investigates the genetics and biology of leukemia through the use of relevant mouse and cell line models. Specific goals include: (1) to determine the biochemical mechanisms governing growth, survival and differentiation of leukemia cells; (2) to identify genes and biochemical pathways that are regulated differentially in leukemia versus normal cells; (3) to measure the growth of distinct populations of normal and leukemia bone marrow cells in vivo and in vitro; (4) to harvest murine bone marrow and embryonic fetal livers for transplantation into irradiated recipients; (5) to transplant genetically modified hematopoietic cells into recipient mice; (6) to perform genetic analysis on mouse tissues; and, (7) to administer chemotherapeutic agents, radiation, and new experimental treatments to mice. This SRA 1 position is for an individual who will work on leukemia research in mouse models and cell lines. SRA duties will include: cell/tissue culture, biochemical assays (western blotting, immunoprecipitation, qPCR), DNA and RNA extraction and manipulation, PCR and agarose gel electrophoresis, generation of recombinant retroviruses/lentiviruses and transduction of hematopoietic cells, flow cytometry, maintenance of accurate and complete records, organization and maintenance of mouse colonies (including computerized record-keeping, breeding, monitoring and genotyping), and experimental procedures using laboratory mice (blood sampling, intravenous/intraperitoneal injection, dissection, analysis of harvested tissues).